Immunosuppressive effect of purified pyocyanine pigment on T-lymphocytes viability against experimental infection with hydatid cyst protoscolices

The effect of pyocyanine pigment, which was isolated and purified from Pseudomonas aeruginosa, on specific lymphocytes viability inside the body of white male Balb/c mice against the experimental secondary hydatidosis and the infectivity of protoscolices was studied in comparison with negative control mice groups, phosphate buffered saline [PBS] and positive control group [immunoferon]. Four groups of male Balb/c mice were intraperitoneally [IP] inoculated with four purified concentrations of pyocyanine [25, 50, 75, 100 micro m/ml]. Seven days later, they were given the same concentrations as a booster dose of the pigment, then 7 days later they were intraperitoneally infected with 2000 protoscoleces/ mL [PBS] as a challenge dose. The fifth group was intraperitoneally inoculated with 1ml of sterile PBS and used as a negative control group, while the sixth group was intraperitoneally inoculated with 100 micro mg/ml immunoferon and received the challenge dose of 2000 protoscoleces/ ml PBS and served as the positive control group. The concentrations of 50, 75 and 100 micro m/ml of this pigment had suppressive effect on these specific immune response cells. This effect was statistically significant [p<0.01] after six weeks from the challenge dose with intraperitoneal protoscolices infection. This effect revealed that the protoscolices infectivity increased due to suppression viability of T lymphocytes, while the immunoferon showed a significant stimulation of these specific cellular cells, which decrease the protoscolices infectivity in comparison with higher pigment concentrations. Pyocyanine is a toxic pigment causing suppression of T-cells activity, especially at higher concentrations which allow protoscolices development and growth

Effect of purified 1-hydroxyphenazine pigment on active and total T rosette formation against secondary hydatidosis

The effect of 1-hydroxyphenazine pigment which was isolated and purified from Pseudomonas aeruginosa on specific immune response T cells inside the body of white male BALB/C mice against experimental secondary hydatidosis and the infectivity of protoscoleces was studied. The aim of this study was to find out the effect of this phenazine pigment [1-hydroxyphenazine] on one of the specific cell-mediated reaction against experimental hyadatidosis. In vivo which may affect the infectivity of hydatid cyst protoscoleces. Six mice groups were used in this research, four of them were injected with[Primary and booster doses] of four concentrations of purified l-hydroxyphenazine pigment [25, 50, 75 and 100 micro mole/ml] isolated from Pseudomonas areuginosa and then injected with 2000 protoscoleces /ml as a challenge dose after 7 days, active and total T rosette were calculated, and after 25 weeks the infectivity of prtoscoleces were studied in relative with the numbers and diameters of hydatid cysts which formed in vitro. In comparison with negative control mice groups [P.B.S.] the results showed that the higher purified concentrations [50, 75 and 100 micro mole/ml] of the pigment had suppressive effect on the specific immune response T cells and this effect was highly significant [P<0.0l] after 6 weeks from challenge dose with protoscoleces intraperitoneally [l.P] against this pigment. This effect reflects that the protoscoleces infectivity was increased due to suppression of T rosette formation activity of T lymphocytes while the mitogen Phytohaemagglutinin [PHA] showed a significant stimulation of the specific cellular response which decrement protoscoleces infectivity in comparison with higher pigment concentration l-hydroxyphenazine is a toxic pigment [dose dependent] causing decrementation of T cells activity especially at higher concentrations which allow protoscoleces development and growth